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flexable mouse igg2a labeling kit  (Proteintech)


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    Proteintech flexable mouse igg2a labeling kit
    (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
    Flexable Mouse Igg2a Labeling Kit, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flexable+mouse+igg2a+labeling+kit/FITC+Mouse+IgG2a+Isotype+Control/pmc12680350-298-11-16
    Average 96 stars, based on 153 article reviews
    flexable mouse igg2a labeling kit - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion"

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1013755

    (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control IgG values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
    Figure Legend Snippet: (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control IgG values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.

    Techniques Used: RNA Immunoprecipitation, Transfection, Expressing, Infection, Immunoprecipitation, Synthesized, Control, Derivative Assay, Reverse Transcription, Amplification, Purification, Modification, Magnetic Beads

    Related Articles

    Labeling:

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. For dsRNA detection, mouse anti-dsRNA Ab was directly labeled using a FlexAble mouse IgG2a labeling kit (Proteintech). .. PLA signals were visualized by confocal microscopy, and the number of PLA foci was quantified using the Analyze Particle plug-in in ImageJ software.



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    flexable mouse igg2a labeling kit  (Proteintech)
    96
    Proteintech flexable mouse igg2a labeling kit
    (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
    Flexable Mouse Igg2a Labeling Kit, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flexable+mouse+igg2a+labeling+kit/FITC+Mouse+IgG2a+Isotype+Control/pmc12680350-298-11-16
    Average 96 stars, based on 1 article reviews
    flexable mouse igg2a labeling kit - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control IgG values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.

    Journal: PLOS Pathogens

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion

    doi: 10.1371/journal.ppat.1013755

    Figure Lengend Snippet: (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control IgG values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.

    Article Snippet: For dsRNA detection, mouse anti-dsRNA Ab was directly labeled using a FlexAble mouse IgG2a labeling kit (Proteintech).

    Techniques: RNA Immunoprecipitation, Transfection, Expressing, Infection, Immunoprecipitation, Synthesized, Control, Derivative Assay, Reverse Transcription, Amplification, Purification, Modification, Magnetic Beads